WHY CHOOSE TIDECELL^®

• 3D In-vivo-like Culture Environment - allows cell-to-cell contact and a large surface area to footprint ratio for culturing cells
• True Linear Scalability - Same parameters are used from 0.1L packed bed volume lab scale for seed preparation, all the way up to 5,000L packed bed volume production scale, eliminating lengthy developmental processes and parameter optimization common in scaling up in microcarriers and / or suspension culture. Unlike other packed bed or hollow fiber systems, seed cells are not prepared in 2D and expanded in 3D, this is 3D to 3D.
• Closed cell harvesting system - TideCell^™ cell harvesting system is a powerful and automated system for harvesting viable cells from 2L to 20L TideCell^™ bioreactors with more than 90% recovery
• 100% nutrient exchange for highest virus titer - Media used for cell growth can be 100% pumped out as waste and 100 % new media is used after virus seeding for virus growth. This has higher yields for VLPs compared to stirred tank which new media can only be perfused in.
• Hybrid System - Can be configured for either single use or combination of single use & autoclavable vessels.
• Difficult Viruses - Parameters such as temperature, pH and oxygen levels are automatically monitored and adjusted to cater to specific virus requirements. TideCell^™ also has zero shear stress for stress sensitive viruses and is capable of harvesting using an automated harvesting system for non-secreted viruses.
• Even seeding - Unlike other packed bed systems, single use TideCell catridges allow even seeding within the packed bed itself via our single use cartridges.
• Isolator Capability - CelCradle/ TideCell^® can be easily integrated inside an Esco Pharma isolator in case the application requires BSL3/4 environment or for Autologous / Allogeneic cell & gene therapy. Controls can be fully integrated into 1 common HMI with Esco BioVap (Hydrogen peroxide biodecontamination or Ammonia system for 6 log reduction in bioburden and/or Esco Autoclave mounted on the side).
• cGMP References - TideCell is used in current good manufacturing practice (cGMP) Facilities for both commercial human and animal vaccines that are certified to FDA, eucGMP, Japanese Industrial Standard (JIS), Pharmaceutical Inspection Co-operation Scheme (PIC/s) standards.
• PAT/IPQC - Process Analytical Techniques (PAT) and In Process Quality Control (IPQC) via patented single use sampling method of BioNoc II allows IPQC for cell counting during production unlike other packed bed bioreactors which need to be dis-assembled during process development.
• In Situ Configurable - Batch, Fed-Batch, Perfusion, continuous processing and parameters for low temperature, anaerobic/hypoxic conditions, single use/autoclavable as well as mixing, feed & harvest tanks with auto switch pumps can be easily configured in-situ via Wonderware. These are easily programmed with minimal training without coding background.
• SCADA enabled with 21 CFR part 11 compliance and CSV - Esco Healthcare factories globally in Singapore/USA/UK provide Supervisory Control and Data Acquisition (SCADA) or Distributed Control System (DCS) Configuration and deployment with computer system validation (CSV). We are well versed in DeltaV, Pcs7 by default Scada system deployed on VacciXcell adherent single use platform is using Wonderware running on Siemens programmable logic controller (PLC). Siemens human machine interface (HMI) can be provided for standalone non SCADA or non-multi-functional dedicated bioprocessing applications
• International support - Esco TideCell^™ is sold & serviced globally by Esco’s 25 direct sales/service offices, factories and trained sales & service partners.