Most cell lines used for biologics production were at one point in time adherent in nature. Progressively, cell lines were engineered from adherent into suspension to make scaling up easier because there was no linearly scalable bioprocess method for adherent cell lines.
However, not all bioprocessing facilities and/or industries have access to suspension-adapted (proprietary) cell lines, especially in developing countries and countries new to bioprocessing. Moreover, some cell lines such as Vero cells or mesenchymal stem cells cannot be easily adapted into suspension.
Microcarriers, traditionally used to scale-up adherent cell cultures, generally require extensive process development time and resources, especially for those new to bioprocessing.
The Tide Motion is a bioprocess platform that eliminates this bottleneck to scaling up adherent cell culture. It provides a single-use adherent cell culture bioprocessing platform with closed and automated cell harvesting for seed preparation (0.1 L packed bed volume) to production (5,000 L packed bed volume: bioequivalent to a 50,000 L suspension culture) all in the same vessel.
The Tide Motion has the highest kLa (volumetric mass-transfer coefficient) amongst all bioprocessing methods and has unlimited oxygenation via its dual oxygenation method.
Tide Motion culture have extremely low cell and DNA debris because of extremely low shear stress with its gentle 2mm/s up-and-down tide motion. It is extremely quick to scale-up (by simply upgrading to the next larger Tide Motion bioreactor). This simplifies downstream processing and lowers costs, making it ideally suited for emergency situations such as pandemics.
The Tide Motion is linearly scalable. This makes it extremely simple to translate R&D into the clinic and commercial production without the usual complex process development required for scaling-up.