Large Scale 3D Bioreactor Technology: Linear Scale Up of Mesenchymal Stromal Cell Expansion with TideXcell™ System


Mesenchymal stromal cell (MSC) therapy requirements can range from several to over 300 million cells per dosage. Conventional expansion of MSCs on plasticware is impractical when larger dosages of over 50 million cells are required. The use of bioreactors combining scaling-up ability, process control and automation is an effective solution to overcome this difficulty, however, many bioreactors face issues in supporting MSC cultures due to complications in balancing the need for adequate mixing of media whilst retaining minimal shear stress, and achieving the ability to separate cells from micro/macro carriers with both high cell yield and viability.

Tide Motion bioreactors are a robust and scalable microcarrier platform to meet the rigorous demands of clinical therapies, working in tandem with the TideXcell™ Harvesting System (TXLHS) to provide automated, high efficiency packed bed cell harvesting.