MRC-5 Human DIploid Cell Culture


MRC-5 diploid cells are an extremely challenging cell line to culture to high densities in solid 3-D matrices. Given their wide application in production of an array of human vaccines, (JordanandSandig,2014) we have optimized the culture conditions using our Tide Motion Bioreactors (CelCradle™-500A) which use BioNOC™ II macrocarriers as the matrix. These cells have been cultured to high densities in these matrices that mimic a 3-D in vivo environment. Cells have been grown to approximately 19-fold the seeding densities.