Optimizing Large-Scale Expansion of Mesenchymal Stem Cells in 3D Tide Motion Bioreactors


The future cell therapy demands high quantities of mesenchymal stem cells (MSCs) ranging from 10 million to more than 200 million cells per dosage. Conventional expansion of MSCs on plastic wares become impractical when large dosages of more than 50 million cells are required. The use of bioreactors which combines scaling-up ability, process control and automation is the primary solution for this need. Many bioreactors are facing issues in supporting MSC cultures due to complications in balancing the need for proper mixing of media with the need to extremely low shear stress as well as the inability to separate cells from micro/macrocarriers with high cell yield and viability.

Esco Aster has leveraged on the Tide Motion bioreactors to establish a robustand scalable platform using macrocarriers to meet the demands for future clinical therapies. MSCs isolated from different tissues sources were seeded and allowed to expand within PET macrocarriers. Throughout culture periods, cell culture conditions were monitored, with bioprocess parameters such as glucose consumption and pH levels measured to ensure proper scale-up. Key issues such as cell seeding densities, media culturing conditions and improved bioprocess parameters needed for optimal stem cell systems were studied in our system. Overall, we present our process optimization with quality controls and release criteria of functional and phenotypic characteristics for the translation of academic/industrial R&D into bench scale for future clinical trials and commercialization process.